小分子生产合成菌群综述：分工、互作与工程控制

图示信息：Figure 1 Product Microbe II Intermediate Microbe I Substrate Microbe III + (a) (b) Effect Ecological Metabolic Application No interaction No common metabolities Amensalism Waste product inhibition Competition Substrate competition Predation Food chain with waste product inhibition 0/0 0/- -/- +/- 0/+ +/+ Commensalism Food chain Cooperation Mutualism or cross-feeding Current Opinion in Biotechnology (a) Schematic representation of basic motifs of metabolite-based microbial interactions (adapted from Grosskopf and Soyer 2014). with positive (+), negative (
) or neutral (0) interactions: no interaction (0/0), amensalism (0/
) for inhibition of a subpopulation by a waste product of another subpopulation, competition (
/
) for a common nutrient required by both microbial subpopulations, predation/parasitism (+/
) as in linear food chains with inhibition of the first subpopulation by a waste product of the second subpopulation, commensalism (0/+) in linear food chains, and mutualism/cooperation (+/+) as in syntrophic linear food chains. Microorganisms (squares) and metabolites (circles) and stimulating (pointed arrows) and inhibitory (blunt arrows) interactions are depicted. (b) Schemati

论文结果 / 观点：该图把群落互作落实到代谢物、电子或营养物交换，是理解共培养功能涌现的关键证据。

研究意义 / 边界：这张图用于支持作者的概念框架或案例归纳；实际迁移到其他系统时，仍需结合成员来源、环境条件、稳定性和功能验证。

来源：1-s2.0-S0958166919300801-main.pdf，PDF 第 2 页；图像来自 PDF 内部可匹配 Figure caption 的图像块。

图示信息：Figure 2 C. glutamicum (α-amylase-negative, L-lysine overproducing) E. coli (α-amylase secreting, L-lysine auxotrophic) Starch Glucose L-lysine L-lysine cadaverine L-pipecolic acid 5μm 5μm 5μm Co-culture for L-lysine production Co-culture for L-pipecolic acid production Co-culture for cadaverine production Current Opinion in Biotechnology Division of labor between substrate conversion (starch hydrolysis) and production (L-lysine, cadaverine and L-pipecolic acid from glucose) in three different synthetic mutualistic E. coli–C. glutamicum consortia (adapted from Sgobba et al. [20]). The fluorescence microscopy pictures showed three E. coli–C. glutamicum consortia in which C. glutamicum cells express the red fluorescence protein Crimson, and E. coli cells express green GfpUV. Each consortium was designed to produce either L-lysine, L- pipecolic acid or cadaverine. www.sciencedirect.com Current Opinion in Biotechnology 2020, 62:72–79

论文结果 / 观点：该图支撑正文关于合成菌群设计、构建、验证或应用边界的核心论证。

研究意义 / 边界：这张图用于支持作者的概念框架或案例归纳；实际迁移到其他系统时，仍需结合成员来源、环境条件、稳定性和功能验证。

来源：1-s2.0-S0958166919300801-main.pdf，PDF 第 4 页；图像来自 PDF 内部可匹配 Figure caption 的图像块。

图示信息：Figure 3 Malonate Glucose, glycerol, xylose Flavanones Flavan-3-ols Anthocyanins Phenylpropanoic acids E. coli converting phenylpropanoic acids and malonate to flavonones E. coli converting flavanones to flavan-3-ols E. coli converting flavan-3-ols to anthocyanins glucose, glycerol and xylose utilizing E. coli producing phenylpropanoic acids Current Opinion in Biotechnology A linear biosynthetic pathway divided over four E. coli strains in a synthetic polyculture (adapted from Jones et al. [36]). The consortium consisted of a strain (brown) producing phenylpropanoic acids from a blend of glucose, xylose, and gylcerol, a strain (red) converting malonate into flavanone, a strain (blue) converting flavanones into flavan-3-ols and a strain (green) converting flavan-3 -ols to anthocyanins. www.sciencedirect.com Current Opinion in Biotechnology 2020, 62:72–79

论文结果 / 观点：该图支撑正文关于合成菌群设计、构建、验证或应用边界的核心论证。

研究意义 / 边界：这张图用于支持作者的概念框架或案例归纳；实际迁移到其他系统时，仍需结合成员来源、环境条件、稳定性和功能验证。

来源：1-s2.0-S0958166919300801-main.pdf，PDF 第 6 页；图像来自 PDF 内部可匹配 Figure caption 的图像块。

图示信息：Figure 4 Glucose Xylose Caffeic acid Salvianic acid A Rosmarinic acid Glucose-negative E.coli producing salvianic acid A Xylose-negative E. coli converting caffeic acid and salvianic acid A to rosmarinic acid Glucose-negative E.coli producing caffeic acid Current Opinion in Biotechnology A biosynthetic pathway with two converging branches divided over three E. coli strains in a synthetic polyculture (adapted from Li et al. [37]). The consortium consisted of a strain (blue) producing caffeic acid from xylose, a strain (brown) producing salvianic acid A from xylose and a strain (green) growing in glucose and converting caffeic acid and salvianic acid A into rosmarinic acid. Current Opinion in Biotechnology 2020, 62:72–79 www.sciencedirect.com

论文结果 / 观点：该图支撑正文关于合成菌群设计、构建、验证或应用边界的核心论证。

研究意义 / 边界：这张图用于支持作者的概念框架或案例归纳；实际迁移到其他系统时，仍需结合成员来源、环境条件、稳定性和功能验证。

来源：1-s2.0-S0958166919300801-main.pdf，PDF 第 7 页；图像来自 PDF 内部可匹配 Figure caption 的图像块。